THE OXIDATION OF PHOSPHORYLATED AND NON-PHOSPHORYLATED SUGARS BY MAMMALIAN LIVER
نویسندگان
چکیده
منابع مشابه
The oxidation of phosphorylated and non-phosphorylated sugars by mammalian liver.
In 1928 Miiller (1) reported the presence of a new enzyme, a glucose oxidase, in press-juice of Aspergillus niger. He subsequently suggested that it might be a dehydrogenase (2) ; he later showed that it catalyzed the oxidation of glucose to gluconic acid (3). Harrison in 1931 obtained glucose dehydrogenase from acetone-dried mammalian liver by saturating a water extract with ammonium sulfate (...
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Seven different forms of the enzyme COMT (catechol O-methyltransferase) were found in isolated rat hepatocytes by two-dimensional gel electrophoresis and immunoblotting: five small variants (S-COMT) and two large variants (L-COMT). The identities of these COMT forms were verified by tryptic fingerprinting using MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS, and by am...
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Heterogeneity among the odontoblast-specific, highly phosphorylated acidic protein dentine phosphoprotein (DPP) obtained from different species has been reported by several investigators. In the present study, the apparent molecular-mass variations in rabbit and mouse DPP were investigated. Extracellular matrix (ECM) DPPs were isolated and characterized. Primary gene products, before post-trans...
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An HPLC method for the separation and analysis of phosphorylated sugars is presented. Ion-exchange chromatography coupled to indirect ultraviolet detection has produced good resolution and sensitivity. Fructose 6-P, glucose 6-P, ribose 5-P, 3-phosphoglyceric acid, ribulose 1,5-P(2), fructose 1,6-P(2), and sedoheptulose 1,7-P(2) can be separated at a sensitivity down to 10 nanomoles. The system ...
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XRCC1 plays a central role in mammalian single-strand break repair. Although it has no enzymatic activity of its own, it stimulates the activities of polynucleotide kinase/phosphatase (PNKP), and this function is enhanced by protein kinase CK2 mediated phosphorylation of XRCC1. We have previously shown that non-phosphorylated XRCC1 stimulates the kinase activity of PNKP by increasing the turnov...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30917-1